Journal: BMC Genomics

Article Title: Benchmarking full-length transcript single cell mRNA sequencing protocols

doi: 10.1186/s12864-022-09014-5

Figure Lengend Snippet: Illustration of the four scRNA-seq protocols applied in this study. A NEBNext® Single Cell/Low Input RNA Library Prep Kit for Illumina (NEB®) followed by NEBNext® uracil excision based (UEB) Final Library Preparation KIT. B SMART-seq® High-Throughput (HT) kit (Takara Bio Inc.) followed by final library preparation using Nextera XT Library preparation kit (Illumina, USA). C Genome & transcriptome sequencing (G&T-seq) followed by final library preparation using Nextera XT Library preparation kit (Illumina, USA). D SMART-seq3 (SS3) followed by final library preparation using Nextera XT Library preparation kit (Illumina, USA) TN5

Article Snippet: In this study, three different SMART-seq full-length protocols; NEBnext® Single Cell/Low Input RNA Library Prep Kit for Illumina (New England Biolabs (NEB®)), SMART-seq® High-Throughput (HT) kit (Takara Bio Inc.), and G&T-seq were performed on T47D cell line for comparison of sensitivity and precision between each protocol.

Techniques: High Throughput Screening Assay, Sequencing

Journal: Nature Communications

Article Title: Cryptic mitochondrial DNA mutations coincide with mid-late life and are pathophysiologically informative in single cells across tissues and species

doi: 10.1038/s41467-025-57286-8

Figure Lengend Snippet: Summary of the public datasets analysed in this study

Article Snippet: The pipeline enables the analysis of scRNA-seq data (e.g., Smart-seq2, CEL-Seq2, 10x Genomics’ ChromiumTM Single Cell 3’ Solution, and 10x Genomics’ ChromiumTM Single Cell 3’ Solution (single-nuclei protocol), and ATAC-seq) but can be adapted to analyse other sequencing data and is a collection of custom-made Shell and Python code.

Techniques: